Preparation of Diphosphopyridine Nucleotide from Bakers’ Yeast
نویسندگان
چکیده
منابع مشابه
DNA polymerases from bakers' yeast.
Two DNA polymerases are present in extracts of commercial bakers' yeast and wild type Saccharomyces cerevisiae grown aerobically to late log phase. Yeast DNA polymerase I and yeast DNA polymerase II can be separated by DEAE-cellulose, hydroxylapatite, and denatured DNA-cellulose chromatography from the postmitochondrial supernatants of yeast lysates. The yeast polymerases are both of high molec...
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In the course of studies of formaldehyde metabolism in yeast (I), an enzyme was found that catalyzed the oxidation of formaldehyde in the presence of glutathione and diphosphopyridine nucleotide. A similar enzyme was discovered independently by Strittmatter and Ball in liver (2). This paper describes the partial purification and some of the properties of yeast formaldehyde dehydrogenase. Some e...
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The older methods for the preparation of diphosphopyridine nucleotide (DPN) were reviewed in 1942 by Schlenk (1). In 1947 Sumner et al. (2) outlined the need for a greatly simplified method for the preparation of this coenzyme. These authors described a procedure which did away with heavy metal fractionation. Recently, Clark et al. (3) made several modifications in the above method. The LePage ...
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Methionyl-tRNA synthetase has been purified from a yeast strain carrying the MESl structural gene on a high copy number plasmid (pFL1). The purified enzyme is a monomer of M, = 85,000 in contrast to its counterpart from Escherichia coli which is a dimer made up of identical subunits (M, = 76,000; Dardel, F., Fayat, G., and Blanquet, S . (1984) J. Bucteriol. 160,1115-1122). The yeast enzyme was ...
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Purified cytochrome c oxidase from bakers’ yeast can be resolved into six polypeptide bands by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The apparent molecular weights of these components are I, 42,000; II, 34,500; III, 23,000; IV, 14,000; V, 12,500; and VI, 9,500. (Although Component V actually consists of two distinct polypeptide species, it will be regarde...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1961
ISSN: 0002-1369
DOI: 10.1080/00021369.1961.10857771